Dozens of local anglers who fish the Gweebarra River claim they have been stopped from using the river by the Northern Region Fisheries Boards.
The row has resulted in a number of charges being brought against 26 anglers for alleged illegal fishing in 2008.
Dozens of fishermen will gather in the village of Doochary today at 10pm to stage their protest.
The protest has been an annual one which began five years ago when locals were excluded from fishing on the Gweebarra by the NRFB.
Chairman of the Donegal Game Angling Federation David McGill said the rights of locals who have fished the river for generations have been eroded.
Mr McGill said that if the NRFB and others think they have gone away, they are badly mistaken.
“We want to send a message to all anglers in Donegal and beyond, that the rights of all anglers such as those using the Gweebarra River, are being greatly eroded and must be resisted,” he said.