The two Japanese Spitz dogs were taken from the garden in Windmill View, Glencar in Letterkenny on Friday afternoon.
The pups were part of a litter of just four dogs which were born just six weeks ago.
The owners told Donegaldaily that they were shocked to find the pups gone.
“I went to see them around 4pm and they were all fine happily playing about rascals at that age.
“And at 5.30 my son came in to play with the pups and said two were missing.
“We all searched every were in the shed around the yard in the house around the estate but they were not to be found. There was no way they could have got out,” said the woman.
The owners are now concerned the dogs may get the Parvo virus as they were still feeding form their mum.
The family also revealed that they had found homes for all four dogs but now had to tell two of the future owners that they will not be getting new dogs.
“I gave my sister and a neighbour the two dogs which were stolen. They are so disappointed my sister told my nieces & nephews they were getting a dog and named him Bruno.
“What sort of dumb people steal six week old puppies form their mother,” asked the woman.